MrkH, a novel c-di-GMP-dependent transcriptional activator controls Klebsiella pneumoniae biofilm formation by regulating type 3 fimbriae expression

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dc.contributor.author Wilksch, Jonathan en_US
dc.contributor.author Yang, Ji en_US
dc.contributor.author Clements, Abigail en_US
dc.contributor.author Gabbe, Jacinta en_US
dc.contributor.author Short, Kirsty en_US
dc.contributor.author Cao, Hanwei en_US
dc.contributor.author James, Catherine en_US
dc.contributor.author Whitchurch, Cynthia en_US
dc.contributor.author Schembri, Mark en_US
dc.contributor.author Chuah, Mary en_US
dc.contributor.author Liang, Zha0-Xun en_US
dc.contributor.author Wijburg, Odilia en_US
dc.contributor.author Jenney, Adam en_US
dc.contributor.author Lithgow, Trevor en_US
dc.contributor.author Strugnell, Richard en_US
dc.contributor.author Cavaliere, Rosy en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-02T07:03:57Z
dc.date.available 2012-02-02T07:03:57Z
dc.date.issued 2011 en_US
dc.identifier 2010003518 en_US
dc.identifier.citation Wilksch Jonathan et al. 2011, 'MrkH, a novel c-di-GMP-dependent transcriptional activator controls Klebsiella pneumoniae biofilm formation by regulating type 3 fimbriae expression', Public Library Science, vol. 7, no. 8, pp. 1002204-1-1002204-22. en_US
dc.identifier.issn 1553-7366 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/14835
dc.description.abstract Klebsiella pneumoniae causes significant morbidity and mortality worldwide, particularly amongst hospitalized individuals. The principle mechanism for pathogenesis in hospital environments involves the formation of biofilms, primarily on implanted medical devices. In this study, we constructed a transposon mutant library in a clinical isolate, K. pneumoniae AJ218, to identify the genes and pathways implicated in biofilm formation. Three mutants severely defective in biofilm formation contained insertions within the mrkABCDF genes encoding the main structural subunit and assembly machinery for type 3 fimbriae. Two other mutants carried insertions within the yfiN and mrkJ genes, which encode GGDEF domain- and EAL domain-containing c-di-GMP turnover enzymes, respectively. The remaining two isolates contained insertions that inactivated the mrkH and mrkI genes, which encode for novel proteins with a c-di-GMP-binding PilZ domain and a LuxR-type transcriptional regulator, respectively. en_US
dc.language en_US
dc.publisher Public Library Science en_US
dc.relation.isbasedon http://dx.doi.org/10.1371/journal.ppat.1002204 en_US
dc.title MrkH, a novel c-di-GMP-dependent transcriptional activator controls Klebsiella pneumoniae biofilm formation by regulating type 3 fimbriae expression en_US
dc.parent Plos Pathogens en_US
dc.journal.volume 7 en_US
dc.journal.number 8 en_US
dc.publocation San Francisco, USA en_US
dc.identifier.startpage 1002204-1 en_US
dc.identifier.endpage 1002204-22 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000068687 en_US
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dc.personcode 0000069845 en_US
dc.personcode 0000069847 en_US
dc.personcode 0000069848 en_US
dc.personcode 0000069856 en_US
dc.personcode 105319 en_US
dc.personcode 01055716 en_US
dc.personcode 103745 en_US
dc.personcode 0000069857 en_US
dc.personcode 0000069858 en_US
dc.personcode 0000069859 en_US
dc.personcode 0000069860 en_US
dc.personcode 0000069862 en_US
dc.personcode 0000069863 en_US
dc.personcode 0000060186 en_US
dc.percentage 50 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US


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