Marine integrons containing novel integrase genes, attachment sites, attI, and associated gene cassettes in polluted sediments from Suez and Tokyo Bays

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dc.contributor.author Elsaied, Hosam en_US
dc.contributor.author Stokes, Harold en_US
dc.contributor.author Kitamura, Keiko en_US
dc.contributor.author Kurusu, Yasurou en_US
dc.contributor.author Kamagata, Yoichi en_US
dc.contributor.author Maruyama, Akihiko en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-02T06:54:16Z
dc.date.available 2012-02-02T06:54:16Z
dc.date.issued 2011 en_US
dc.identifier 2010003442 en_US
dc.identifier.citation Elsaied Hosam et al. 2011, 'Marine integrons containing novel integrase genes, attachment sites, attI, and associated gene cassettes in polluted sediments from Suez and Tokyo Bays', Nature Publishing Group, vol. 5, no. 7, pp. 1162-1177. en_US
dc.identifier.issn 1751-7362 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/14816
dc.description.abstract In order to understand the structure and biological significance of integrons and associated gene cassettes in marine polluted sediments, metagenomic DNAs were extracted from sites at Suez and Tokyo Bays. PCR amplicons containing new integrase genes, intI, linked with novel gene cassettes, were recovered and had sizes from 1.8 to 2.5 kb. This approach uncovered, for the first time, the structure and diversity of both marine integron attachment site, attI, and the first gene cassette, the most efficiently expressed integron-associated gene cassette. The recovered 13 and 20 intI phylotypes, from Suez and Tokyo Bay samples, respectively, showed a highly divergence, suggesting a difference in integron composition between the sampling sites. Some intI phylotypes showed similarity with that from Geobacter metallireducens, belonging to Deltaproteobacteria, the dominant class in both sampling sites, as determined by 16S rRNA gene analysis. Thirty distinct families of putative attI site, as determined by the presence of an attI-like simple site, were recovered. A total of 146 and 68 gene cassettes represented Suez and Tokyo Bay unsaturated cassette pools, respectively. Gene cassettes, including a first cassette, from both sampling sites encoded two novel families of glyoxalase/bleomycin antibiotic-resistance protein. Gene cassettes from Suez Bay encoded proteins similar to haloacid dehalogenases, protein disulfide isomerases and death-oncuring and plasmid maintenance system killer proteins. en_US
dc.language en_US
dc.publisher Nature Publishing Group en_US
dc.relation.isbasedon http://dx.doi.org/10.1038/ismej.2010.208 en_US
dc.title Marine integrons containing novel integrase genes, attachment sites, attI, and associated gene cassettes in polluted sediments from Suez and Tokyo Bays en_US
dc.parent ISME Journal en_US
dc.journal.volume 5 en_US
dc.journal.number 7 en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 1162 en_US
dc.identifier.endpage 1177 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060000 en_US
dc.personcode 0000068707 en_US
dc.personcode 105741 en_US
dc.personcode 0000068708 en_US
dc.personcode 0000068709 en_US
dc.personcode 0000068710 en_US
dc.personcode 0000068711 en_US
dc.percentage 100 en_US
dc.classification.name Biological Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords integrons; gene cassettes; metagenome; polluted marine sediment en_US
dc.staffid en_US


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