The preparation and use of biotinylated trypsin in western blotting for the detection of trypsin inhibitory proteins

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dc.contributor.author Melrose J en_US
dc.contributor.author Rodgers Kenneth en_US
dc.contributor.author Ghosh Peter en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-02T05:38:08Z
dc.date.available 2012-02-02T05:38:08Z
dc.date.issued 1994 en_US
dc.identifier 2010000603 en_US
dc.identifier.citation Melrose J, Rodgers Kenneth, and Ghosh Peter 1994, 'The preparation and use of biotinylated trypsin in western blotting for the detection of trypsin inhibitory proteins', Academic Press Inc Jnl-Comp Subscriptions, vol. 222, no. 1, pp. 34-43. en_US
dc.identifier.issn 0003-2697 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/14667
dc.description.abstract Methods were developed for the preparation of biotinylated trypsin of high specific activity. This was used as a probe for the detection of serine proteinase inhibitory proteins which had been separated by sodium dodecyl sulfate-polyacrylamide gradient s en_US
dc.language en_US
dc.publisher Academic Press Inc Jnl-Comp Subscriptions en_US
dc.relation.isbasedon http://dx.doi.org/10.1006/abio.1994.1450 en_US
dc.title The preparation and use of biotinylated trypsin in western blotting for the detection of trypsin inhibitory proteins en_US
dc.parent Analytical Biochemistry en_US
dc.journal.volume 222 en_US
dc.journal.number 1 en_US
dc.publocation San Diego, USA en_US
dc.identifier.startpage 34 en_US
dc.identifier.endpage 43 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 030100 en_US
dc.personcode 0000035041;111642;0000017324 en_US
dc.percentage 000050 en_US
dc.classification.name Analytical Chemistry en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:A1994PM43900006 en_US
dc.description.keywords Human Articular-Cartilage; Sodium Dodecyl-Sulfate; Polyacrylamide-Gel-Electrophoresis; Human Intervertebral-Disk; Serine-Proteinase; Protease Inhibitors; Molecular-Weight; Emphysema; Purification; Assay en_US
dc.staffid The Heart Research Institute;University of Sydney en_US


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