A continuous fluorescence assay for the study of P-glycoprotein-mediated drug efflux using inside-out membrane vesicles

UTSePress Research/Manakin Repository

Search UTSePress Research


Advanced Search

Browse

My Account

Show simple item record

dc.contributor.author Bebawy, Mary en_US
dc.contributor.author Morris, M B en_US
dc.contributor.author Roufogalis, Basil en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-02T05:35:35Z
dc.date.available 2012-02-02T05:35:35Z
dc.date.issued 1999 en_US
dc.identifier 2010004309 en_US
dc.identifier.citation Bebawy Mary, Morris M B, and Roufogalis Basil 1999, 'A continuous fluorescence assay for the study of P-glycoprotein-mediated drug efflux using inside-out membrane vesicles', Academic Press Inc, vol. 268, no. 2, pp. 270-277. en_US
dc.identifier.issn 0003-2697 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/14662
dc.description.abstract A fluorimetric procedure for assaying the transport activity of P-glycoprotein (P-gp) using a membrane vesicle model has been developed. In this assay methylene blue is incorporated into inside-out vesicles prepared from human acute lymphoblastic leukemic cells resistant to 100 ng z ml21 vinblastine (VBL100) and their sensitive controls. The fluorescence of a fluorescent derivative of colchicine (fluorescein?colchicine) is quenched as the probe is transported across the vesicle membrane. The fluorescein?colchicine transport was found to be dependent on the presence of P-glycoprotein, required ATP, and was inhibited by vanadate and the reversal agent, verapamil, in a dose-dependent manner. Furthermore, the transport was competed against by the P-gp substrates, vinblastine and methotrexate. The transport of fluorescein?colchicine by P-gp was found to be cooperative (n 5 1.23). en_US
dc.language en_US
dc.publisher Academic Press Inc en_US
dc.relation.isbasedon http://dx.doi.org/10.1006/abio.1998.3087 en_US
dc.title A continuous fluorescence assay for the study of P-glycoprotein-mediated drug efflux using inside-out membrane vesicles en_US
dc.parent Analytical Biochemistry en_US
dc.journal.volume 268 en_US
dc.journal.number 2 en_US
dc.publocation San Diego en_US
dc.identifier.startpage 270 en_US
dc.identifier.endpage 277 en_US
dc.cauo.name GSH.Pharmacy en_US
dc.conference Verified OK en_US
dc.for 030100 en_US
dc.personcode 112474 en_US
dc.personcode 0000069522 en_US
dc.personcode X000240 en_US
dc.percentage 100 en_US
dc.classification.name Analytical Chemistry en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity WOS:000079508100014 en_US
dc.description.keywords fluorescein?colchicine; multidrug resistance; P-glycoprotein substrates; methylene blue; inside-out membrane vesicle en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record