Analysis of mercury-containing protein fractions in brain cytosol of the maternal and infant rats after exposure to a low-dose of methylmercury by SEC coupled to isotope dilution ICP-MS

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dc.contributor.author Wang, Meng en_US
dc.contributor.author Feng, Wei-Yue en_US
dc.contributor.author Wang, Hua-Jian en_US
dc.contributor.author Zhang, Yu en_US
dc.contributor.author Li, Jing en_US
dc.contributor.author Li, Bai en_US
dc.contributor.author Zhao, Yu-Liang en_US
dc.contributor.author Chai, Zhi-Fang en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-02T05:34:03Z
dc.date.available 2012-02-02T05:34:03Z
dc.date.issued 2008 en_US
dc.identifier 2010003215 en_US
dc.identifier.citation Wang Meng et al. 2008, 'Analysis of mercury-containing protein fractions in brain cytosol of the maternal and infant rats after exposure to a low-dose of methylmercury by SEC coupled to isotope dilution ICP-MS', Royal Society of Chemistry, vol. 23, no. 8, pp. 1112-1116. en_US
dc.identifier.issn 0267-9477 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/14659
dc.description.abstract The mercury-containing protein fractions in the brain cytosol of the maternal and infant rats after exposure to low-dose of methylmercury chloride (0.3 mg Hg kg 1 day 1) were analyzed by a SEC-ICPMS method with the postcolumn isotope dilution analysis. The enriched spiking including 34S, 65Cu, 67Zn and 198Hg was continuously added into the eluate from the HPLC column and then the isotopediluted fractions were on-line measured by ICP-MS. Therefore, the absolute amounts of sulfur, mercury, zinc and copper in the eluted protein fractions could be attained after calculation of the corresponding peak areas in the mass flow chromatogram. Five mercury-containing protein fractions were monitored in the maternal sample, whereas only one was in the infant sample. The mercury content in the infant sample was about 10 times lower than the one in the maternal sample. In the meantime, two copper- and four zinc-containing fractions were found in both maternal and infant samples. The detection limit for S, Cu, Zn and Hg are 11, 0.1, 1.5 and 0.2 ng, respectively. The results demonstrate that different mercury-containing protein fractions may exist in brain cytosol between maternal and infant rats and the quantitative calculation may be helpful for the toxicological study. en_US
dc.language en_US
dc.publisher Royal Society of Chemistry en_US
dc.relation.isbasedon http://dx.doi.org/10.1039/b802124d en_US
dc.title Analysis of mercury-containing protein fractions in brain cytosol of the maternal and infant rats after exposure to a low-dose of methylmercury by SEC coupled to isotope dilution ICP-MS en_US
dc.parent Journal of Analytical Atomic Spectrometry en_US
dc.journal.volume 23 en_US
dc.journal.number 8 en_US
dc.publocation Cambridge, UK en_US
dc.identifier.startpage 1112 en_US
dc.identifier.endpage 1116 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 030100 en_US
dc.personcode 112014 en_US
dc.personcode 0000068160 en_US
dc.personcode 0000068203 en_US
dc.personcode 0000068204 en_US
dc.personcode 0000068205 en_US
dc.personcode 0000068206 en_US
dc.personcode 0000068185 en_US
dc.personcode 0000068186 en_US
dc.percentage 100 en_US
dc.classification.name Analytical Chemistry en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US


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