Lab-on-a-chip biosensor for glucose based on a packed immobilized enzyme reactor

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dc.contributor.author Blanes, Lucas en_US
dc.contributor.author Mora, Maria en_US
dc.contributor.author Do Lago, Claudimir en_US
dc.contributor.author Ayon, Arturo en_US
dc.contributor.author Garcia, Carlos en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-02T05:33:02Z
dc.date.available 2012-02-02T05:33:02Z
dc.date.issued 2007 en_US
dc.identifier 2010002070 en_US
dc.identifier.citation Blanes Lucas et al. 2007, 'Lab-on-a-chip biosensor for glucose based on a packed immobilized enzyme reactor', Wiley - VCH Verlag GmbH & Co. KGaA, vol. 19, no. 23, pp. 2451-2456. en_US
dc.identifier.issn 1040-0397 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/14657
dc.description.abstract In this work, the development of a packed immobilized enzyme reactor (IMER) and its integration to a capillary electrophoresis microchip is described. The present microchip design differs from others, in the fact that the same design could be used with or without the particles and, just by changing the material used to pack the IMER, different analytes can be detected. The applied procedure involves the separation of the target analyte by capillary electrophoresis (CE), which is then coupled to a post-column IMER that produces H2O2. The H2O2 produced is finally detected downstream at the surface of a working electrode. Glucose was detected above 100 mM by packing particles modified with glucose oxidase at the end of the separation channel. The analytical performance of the microchip-CE has been demonstrated by performing the separation and detection of glucose and noradrenaline. Additions of fructose showed no effect on either the peak position or the peak magnitude of glucose. The microchip-CE-IMER was also used to quantify glucose in carbonated beverages with good agreement with other reports. en_US
dc.language en_US
dc.publisher Wiley - VCH Verlag GmbH & Co. KGaA en_US
dc.relation.isbasedon http://dx.doi.org/10.1002/elan.200704001 en_US
dc.title Lab-on-a-chip biosensor for glucose based on a packed immobilized enzyme reactor en_US
dc.parent Electroanalysis en_US
dc.journal.volume 19 en_US
dc.journal.number 23 en_US
dc.publocation Germany en_US
dc.identifier.startpage 2451 en_US
dc.identifier.endpage 2456 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 039900 en_US
dc.personcode 105015 en_US
dc.personcode 0000066872 en_US
dc.personcode 0000066873 en_US
dc.personcode 0000066874 en_US
dc.personcode 0000066875 en_US
dc.percentage 100 en_US
dc.classification.name Other Chemical Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Capillary electrophoresis, Microchips, Glucose, Immobilized enzyme reactor en_US
dc.staffid en_US


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