Development of a mild mercaptoethanol extraction method for determination of mercury species in biological samples by HPLC-ICP-MS

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dc.contributor.author Wang, Meng en_US
dc.contributor.author Feng, Weiyue en_US
dc.contributor.author Shi, Junwen en_US
dc.contributor.author Zhang, Fang en_US
dc.contributor.author Wang, Bing en_US
dc.contributor.author Zhu, Motao en_US
dc.contributor.author Li, Bai en_US
dc.contributor.author Zhao, Yuliang en_US
dc.contributor.author Chai, Zhifang en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-02T05:06:02Z
dc.date.available 2012-02-02T05:06:02Z
dc.date.issued 2007 en_US
dc.identifier 2010003224 en_US
dc.identifier.citation Wang Meng et al. 2007, 'Development of a mild mercaptoethanol extraction method for determination of mercury species in biological samples by HPLC-ICP-MS', Elsevier Inc, vol. 71, no. 5, pp. 2034-2039. en_US
dc.identifier.issn 0039-9140 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/14604
dc.description.abstract A mild, efficient and convenient extraction method of using 2-mercaptoethanol contained extractant solution combined with an incubator shaker for determination of mercury species in biological samples by HPLC?ICP-MS has been developed. The effects of the concentration of 2-mercaptoethanol, the composition of the extractant solution and the shaking time on the efficiency of mercury extraction were evaluated. The optimization experiments indicated that the quantitative extraction of mercury species from biological samples could be achieved by using 0.1% (v/v) HCl, 0.1% (v/v) 2-mercapoethanol and 0.15% (m/v) KCl extractant solution in an incubator shaker for shaking overnight (about 12 h) at room temperature. The established method was validated by analysis of various biological certified reference materials, including NRCC DOLT-3 (dogfish liver), IAEA 436 (tuna fish), IAEA MA-B-3/TM (garfish filet), IAEA MA-M-2/TM (mussel tissue), GBW 08193 (bovine liver) and GBW 08572 (prawn). The analytical results of the reference materials were in good agreement with the certified or reference values of both methyl and total mercury, indicating that no distinguishable transformation between mercury species had occurred during the extraction and determination procedures. The limit of detection (LOD) for methyl (CH3Hg+) and inorganic mercury (Hg2+) by the method are both as 0.2 gL-1. The relative standard deviation (R.S.D.s) for CH3Hg+ and Hg2+ are 3.0% and 5.8%, respectively. The advantages of the developed extraction method are that (1) it is easy to operate in HPLC?ICP-MS for mercury species determination since the extracted solution can be directly injected into the HPLC column without pH adjustment and (2) the memory effect of mercury in the ICP-MS measurement system can be reduced. en_US
dc.language en_US
dc.publisher Elsevier Inc en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/j.talanta.2006.09.012 en_US
dc.title Development of a mild mercaptoethanol extraction method for determination of mercury species in biological samples by HPLC-ICP-MS en_US
dc.parent Talanta en_US
dc.journal.volume 71 en_US
dc.journal.number 5 en_US
dc.publocation Amsterdam, Netherlands en_US
dc.identifier.startpage 2034 en_US
dc.identifier.endpage 2039 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 030100 en_US
dc.personcode 112014 en_US
dc.personcode 0000068194 en_US
dc.personcode 0000068199 en_US
dc.personcode 0000068184 en_US
dc.personcode 0000068159 en_US
dc.personcode 0000068197 en_US
dc.personcode 0000068206 en_US
dc.personcode 0000068200 en_US
dc.personcode 0000068201 en_US
dc.percentage 100 en_US
dc.classification.name Analytical Chemistry en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Mercury speciation; Mild extraction; HPLC?ICP-MS; Biological sample; Memory effect en_US


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