Resistance To Chloramphenicol In Proteus-Mirabilis By Expression Of A Chromosomal Gene For Chloramphenicol Acetyltransferase

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dc.contributor.author Charles, Ian en_US
dc.contributor.author Harford, S en_US
dc.contributor.author Brookfield, Jfy en_US
dc.contributor.author Shaw, Wv en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:24:48Z
dc.date.available 2011-02-07T06:24:48Z
dc.date.issued 1985 en_US
dc.identifier 2009002873 en_US
dc.identifier.citation Charles Ian et al. 1985, 'Resistance To Chloramphenicol In Proteus-Mirabilis By Expression Of A Chromosomal Gene For Chloramphenicol Acetyltransferase', Amer Soc Microbiology, vol. 164, no. 1, pp. 114-122. en_US
dc.identifier.issn 0021-9193 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13796
dc.description.abstract Proteus mirabilis PM13 is a well-characterized chloramphenicol-sensitive isolate which spontaneously gives rise to resistant colonies on solid media containing chloramphenicol (50 micrograms ml-1) at a plating efficiency of 10(-4) to 10(-5). Such chloramphenicol-resistant colonies exhibit a novel phenotype with respect to chloramphenicol resistance. When a single colony grown on chloramphenicol agar is transferred to liquid medium and grown in the absence of antibiotic for 150 generations, a population of predominantly sensitive cells arises. This mutation-reversion phenomenon has been observed in other Proteus species and Providencia strains, wherein resistance has been shown to be mediated in each case by the enzyme chloramphenicol acetyltransferase. The cat gene responsible for the phenomenon is chromosomal and can be cloned from P. mirabilis PM13 with DNA prepared from cells grown in the absence or the presence of chloramphenicol. Recombinant plasmids which confer resistance to chloramphenicol carry an 8.5-kilobase PstI fragment irrespective of the source of host DNA. The location of the cat gene within the PstI fragment was determined by Southern blotting with a cat consensus oligonucleotide corresponding to the expected amino acid sequence of the active site region of chloramphenicol acetyltransferase, and the direction of transcription was deduced from homology with the type I cat variant. en_US
dc.language en_US
dc.publisher Amer Soc Microbiology en_US
dc.title Resistance To Chloramphenicol In Proteus-Mirabilis By Expression Of A Chromosomal Gene For Chloramphenicol Acetyltransferase en_US
dc.parent Journal Of Bacteriology en_US
dc.journal.volume 164 en_US
dc.journal.number 1 en_US
dc.publocation Washington en_US
dc.identifier.startpage 114 en_US
dc.identifier.endpage 122 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 110800 en_US
dc.personcode 109028 en_US
dc.personcode 0000060133 en_US
dc.personcode 0000060132 en_US
dc.personcode 0000060134 en_US
dc.percentage 100 en_US
dc.classification.name Medical Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:A1985ASA7400017 en_US
dc.description.keywords NA en_US
dc.staffid en_US


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