Gamma irradiation as a biological decontaminant and its effect on common fingermark detection techniques and DNA profiling

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dc.contributor.author Hoile, Rebecca en_US
dc.contributor.author Banos, Connie en_US
dc.contributor.author Colella, Michael en_US
dc.contributor.author Walsh, Simon en_US
dc.contributor.author Roux, Claude en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:24:44Z
dc.date.available 2011-02-07T06:24:44Z
dc.date.issued 2010 en_US
dc.identifier 2009007655 en_US
dc.identifier.citation Hoile Rebecca et al. 2010, 'Gamma irradiation as a biological decontaminant and its effect on common fingermark detection techniques and DNA profiling', Blackwell Publishing Ltd, vol. 55, pp. 171-177. en_US
dc.identifier.issn 0022-1198 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/13788
dc.description.abstract The use of disease-causing organisms and their toxins against the civilian population has defined bioterrorism and opened forensic science up to the challenges of processing contaminated evidence. This study sought to detennine the use of gamma irradiation as an effective biological decontaminant and its effect on the recovery of latent fingennarks from both porous and nonporous items. Test items were contaminated with viable spores marked with latent pIinrs and then decontaminated using a cobalt 60 gamma irradiator. Fingerrnark detection was the focus with standard methods inclurJing 1,2-inrJanedione, ninhydrin, diazafluoren-9-one, and physical developer used during this study. DNA recovery using 20% Chelex extraction and quantitative real-time polymerdse chain reaction was also explored. Gamma irradiation proved effective as a bacterial decontaminant with D-values ranging from 458 to 500 Gy for nonporous items and 797-808 Gy for porous ones. The results demonstrated the successful recovel)' of latent marks and DNA establishing gamma irradiation as a viable decontamination option. en_US
dc.language en_US
dc.publisher Blackwell Publishing Ltd en_US
dc.relation.isbasedon http://dx.doi.org/10.1111/j.1556-4029.2009.01233.x en_US
dc.title Gamma irradiation as a biological decontaminant and its effect on common fingermark detection techniques and DNA profiling en_US
dc.parent Journal of Forensic Sciences en_US
dc.journal.volume 55 en_US
dc.journal.number en_US
dc.journal.number 1 en_US
dc.publocation USA en_US
dc.identifier.startpage 171 en_US
dc.identifier.endpage 177 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 039900 en_US
dc.personcode 109664 en_US
dc.personcode 0000063886 en_US
dc.personcode 10202950 en_US
dc.personcode 02103822 en_US
dc.personcode 960382 en_US
dc.percentage 100 en_US
dc.classification.name Other Chemical Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords forensic science, gamma irradiation, microbial forensics, latent fingennarks, DNA, bioterrorism en_US
dc.staffid 960382 en_US


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