Bacterial membrane vesicles deliver peptidoglycan to NOD1 in epithelial cells

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dc.contributor.author Kaparakis, Maria en_US
dc.contributor.author Turnbull, Lynne en_US
dc.contributor.author Carneiro, L en_US
dc.contributor.author Firth, Steven en_US
dc.contributor.author Coleman, Harry en_US
dc.contributor.author Parkington, Helena en_US
dc.contributor.author Le Bourhis, L en_US
dc.contributor.author Karrar, Abdul en_US
dc.contributor.author Viala, J en_US
dc.contributor.author Mak, Johnson en_US
dc.contributor.author Hutton, Melanie en_US
dc.contributor.author Davies, John en_US
dc.contributor.author Crack, P en_US
dc.contributor.author Hertzog, Paul en_US
dc.contributor.author Philpott, Dana en_US
dc.contributor.author Girardin, Stephen en_US
dc.contributor.author Whitchurch, Cynthia en_US
dc.contributor.author Ferrero, Richard en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:24:34Z
dc.date.available 2011-02-07T06:24:34Z
dc.date.issued 2010 en_US
dc.identifier 2009004733 en_US
dc.identifier.citation Kaparakis Maria et al. 2010, 'Bacterial membrane vesicles deliver peptidoglycan to NOD1 in epithelial cells', Wiley-Blackwell, vol. 12, no. 3, pp. 372-385. en_US
dc.identifier.issn 1462-5814 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/13769
dc.description.abstract Gram-negative bacterial peptidoglycan is specifically recognized by the host intracellular sensor NOD1, resulting in the generation of innate immune responses. Although epithelial cells are normally refractory to external stimulation with peptidoglycan, these cells have been shown to respond in a NOD1-dependent manner to Gram-negative pathogens that can either invade or secrete factors into host cells. In the present work, we report that Gram-negative bacteria can deliver peptidoglycan to cytosolic NOD1 in host cells via a novel mechanism involving outer membrane vesicles (OMVs). We purified OMVs from the Gram-negative mucosal pathogens: Helicobacter pylori, Pseudomonas aeruginosa and Neisseria gonorrhoea and demonstrated that these peptidoglycan containing OMVs upregulated NF-kappaB and NOD1-dependent responses in vitro. These OMVs entered epithelial cells through lipid rafts thereby inducing NOD1-dependent responses in vitro. Moreover, OMVs delivered intragastrically to mice-induced innate and adaptive immune responses via a NOD1-dependent but TLR-independent mechanism. Collectively, our findings identify OMVs as a generalized mechanism whereby Gram-negative bacteria deliver peptidoglycan to cytosolic NOD1. We propose that OMVs released by bacteria in vivo may promote inflammation and pathology in infected hosts. en_US
dc.language en_US
dc.publisher Wiley-Blackwell en_US
dc.relation.hasversion Accepted manuscript version en_US
dc.relation.isbasedon http://dx.doi.org/10.1111/j.1462-5822.2009.01404.x en_US
dc.title Bacterial membrane vesicles deliver peptidoglycan to NOD1 in epithelial cells en_US
dc.parent Cellular Microbiology en_US
dc.journal.volume 12 en_US
dc.journal.number 3 en_US
dc.publocation New York en_US
dc.identifier.startpage 372 en_US
dc.identifier.endpage 385 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
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dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords en_US


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