Bacterial membrane vesicles deliver peptidoglycan to NOD1 in epithelial cells

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Show simple item record Kaparakis, Maria en_US Turnbull, Lynne en_US Carneiro, L en_US Firth, Steven en_US Coleman, Harry en_US Parkington, Helena en_US Le Bourhis, L en_US Karrar, Abdul en_US Viala, J en_US Mak, Johnson en_US Hutton, Melanie en_US Davies, John en_US Crack, P en_US Hertzog, Paul en_US Philpott, Dana en_US Girardin, Stephen en_US Whitchurch, Cynthia en_US Ferrero, Richard en_US
dc.contributor.editor en_US 2011-02-07T06:24:34Z 2011-02-07T06:24:34Z 2010 en_US
dc.identifier 2009004733 en_US
dc.identifier.citation Kaparakis Maria et al. 2010, 'Bacterial membrane vesicles deliver peptidoglycan to NOD1 in epithelial cells', Wiley-Blackwell, vol. 12, no. 3, pp. 372-385. en_US
dc.identifier.issn 1462-5814 en_US
dc.identifier.other C1 en_US
dc.description.abstract Gram-negative bacterial peptidoglycan is specifically recognized by the host intracellular sensor NOD1, resulting in the generation of innate immune responses. Although epithelial cells are normally refractory to external stimulation with peptidoglycan, these cells have been shown to respond in a NOD1-dependent manner to Gram-negative pathogens that can either invade or secrete factors into host cells. In the present work, we report that Gram-negative bacteria can deliver peptidoglycan to cytosolic NOD1 in host cells via a novel mechanism involving outer membrane vesicles (OMVs). We purified OMVs from the Gram-negative mucosal pathogens: Helicobacter pylori, Pseudomonas aeruginosa and Neisseria gonorrhoea and demonstrated that these peptidoglycan containing OMVs upregulated NF-kappaB and NOD1-dependent responses in vitro. These OMVs entered epithelial cells through lipid rafts thereby inducing NOD1-dependent responses in vitro. Moreover, OMVs delivered intragastrically to mice-induced innate and adaptive immune responses via a NOD1-dependent but TLR-independent mechanism. Collectively, our findings identify OMVs as a generalized mechanism whereby Gram-negative bacteria deliver peptidoglycan to cytosolic NOD1. We propose that OMVs released by bacteria in vivo may promote inflammation and pathology in infected hosts. en_US
dc.language en_US
dc.publisher Wiley-Blackwell en_US
dc.relation.hasversion Accepted manuscript version en_US
dc.relation.isbasedon en_US
dc.title Bacterial membrane vesicles deliver peptidoglycan to NOD1 in epithelial cells en_US
dc.parent Cellular Microbiology en_US
dc.journal.volume 12 en_US
dc.journal.number 3 en_US
dc.publocation New York en_US
dc.identifier.startpage 372 en_US
dc.identifier.endpage 385 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000061666 en_US
dc.personcode 103744 en_US
dc.personcode 0000061667 en_US
dc.personcode 0000061668 en_US
dc.personcode 0000061669 en_US
dc.personcode 0000061670 en_US
dc.personcode 0000061671 en_US
dc.personcode 0000061672 en_US
dc.personcode 0000061673 en_US
dc.personcode 0000041916 en_US
dc.personcode 0000061674 en_US
dc.personcode 0000051668 en_US
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dc.personcode 0000061678 en_US
dc.personcode 103745 en_US
dc.personcode 0000061679 en_US
dc.percentage 100 en_US Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords en_US

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