Cloning and Characterization of the aroA Gene from Mycobacterium tuberculosis

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Show simple item record Garbe, Thomas en_US Jones, Christopher en_US Charles, Ian en_US Dougan, Gordon en_US Young, Douglas en_US
dc.contributor.editor en_US 2011-02-07T06:24:11Z 2011-02-07T06:24:11Z 1990 en_US
dc.identifier 2009004434 en_US
dc.identifier.citation Garbe Thomas et al. 1990, 'Cloning and Characterization of the aroA Gene from Mycobacterium tuberculosis', Amer Soc Microbiology, vol. 172, no. 12, pp. 6774-6782. en_US
dc.identifier.issn 0021-9193 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract The aroA gene from Mycobacterium tuberculosis has been cloned by complementation of an aroA mutant of Escherichia coli after lysogenization with a recombinant DNA library in the lambda gt11 vector. Detailed characterization of the M. tuberculosis aroA gene by nucleotide sequencing and by immunochemical analysis of the expressed product indicates that it encodes a 5-enolpyruvylshikimate-3-phosphate synthase that is structurally related to analogous enzymes from other bacterial, fungal, and plant sources. The potential use of the cloned gene in construction of genetically defined mutant strains of M. tuberculosis by gene replacement is proposed as a novel approach to the rational attenuation of mycobacterial pathogens and the possible development of new antimycobacterial vaccines. en_US
dc.language en_US
dc.publisher Amer Soc Microbiology en_US
dc.title Cloning and Characterization of the aroA Gene from Mycobacterium tuberculosis en_US
dc.parent Journal Of Bacteriology en_US
dc.journal.volume 172 en_US
dc.journal.number 12 en_US
dc.publocation Washington DC, USA en_US
dc.identifier.startpage 6774 en_US
dc.identifier.endpage 6782 en_US SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 110800 en_US
dc.personcode 0000061437 en_US
dc.personcode 0000061438 en_US
dc.personcode 109028 en_US
dc.personcode 0000061403 en_US
dc.personcode 0000061439 en_US
dc.percentage 100 en_US Medical Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid en_US

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