A New Microsphere-based Immunofluorescence Assay For Antibodies To Membrane-associated Antigens

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dc.contributor.author Wilson, Merilyn en_US
dc.contributor.author Mulligan, Sp en_US
dc.contributor.author Raison, Robert en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:24:02Z
dc.date.available 2011-02-07T06:24:02Z
dc.date.issued 1988 en_US
dc.identifier 2006014251 en_US
dc.identifier.citation Wilson Merilyn, Mulligan Sp, and Raison Robert 1988, 'A New Microsphere-based Immunofluorescence Assay For Antibodies To Membrane-associated Antigens', Elsevier Science Bv, vol. 107, no. 2, pp. 231-237. en_US
dc.identifier.issn 0022-1759 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13704
dc.description.abstract The screening of panels of hybridoma supernatants for specific secreted monoclonal antibodies is often achieved by cellular immunofluorescent staining and flow cytometric analysis. In some circumstances such assays are difficult because the required antigen-bearing cell population is not suitable for use in flow cytometry, has limited cell cycle expression or poor in vitro growth. A method is presented here that provides a solution to these difficulties. A system was developed, using polyacrylamide microspheres coupled with cell membranes, which permitted the production of an easily stored, standardised antigen source which could be used in subsequent flow cytometric assays. Studies comparing the binding of antibodies to whole cells and cell membrane-coupled microspheres indicate a strong qualitative and quantitative correlation in the expression of surface antigens. It is shown here that membrane antigens can be stored coupled to microspheres for months and still retain good reactivity with the appropriate antibodies. This same technique could be used in studies of antigens other than those of the mammalian cell membrane - for example, membrane antigens of sub-cellular organelles such as the mitochondrion and endoplasmic reticulum, plant or bacterial membrane antigens, or antigens not associated with membranes such as viral protein en_US
dc.language en_US
dc.publisher Elsevier Science Bv en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/0022-1759(88)90223-2 en_US
dc.title A New Microsphere-based Immunofluorescence Assay For Antibodies To Membrane-associated Antigens en_US
dc.parent Journal Of Immunological Methods en_US
dc.journal.volume 107 en_US
dc.journal.number 2 en_US
dc.publocation Amsterdam en_US
dc.identifier.startpage 231 en_US
dc.identifier.endpage 237 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 110700 en_US
dc.personcode 921106 en_US
dc.personcode 0000035072 en_US
dc.personcode 890404 en_US
dc.percentage 100 en_US
dc.classification.name Immunology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:A1988M423300010 en_US
dc.description.keywords Membrane-microsphere conjugate; Flow cytometry; Immunofluorescence assay en_US
dc.staffid en_US
dc.staffid 890404 en_US

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