Molecular cloning and characterization of the aroD gene encoding 3-dehydroquinase from Salmonella typhi

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dc.contributor.author Servos, Spiros en_US
dc.contributor.author Chatfield, Steve en_US
dc.contributor.author Hone, David en_US
dc.contributor.author Levine, Myron en_US
dc.contributor.author Dimitriadis, George en_US
dc.contributor.author Pickard, Derek en_US
dc.contributor.author Dougan, Gordon en_US
dc.contributor.author Fairweather, Neil en_US
dc.contributor.author Charles, Ian en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:21:18Z
dc.date.available 2011-02-07T06:21:18Z
dc.date.issued 1991 en_US
dc.identifier 2009004802 en_US
dc.identifier.citation Servos Spiros et al. 1991, 'Molecular cloning and characterization of the aroD gene encoding 3-dehydroquinase from Salmonella typhi', Soc General Microbiology, vol. 137, no. 1, pp. 147-152. en_US
dc.identifier.issn 0022-1287 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13397
dc.description.abstract The aroD gene from Salmonella typhi, encoding 5-dehydroquinate hydrolyase (3-dehydroquinase), has been cloned into Escherichia coli and the DNA sequence determined. The aroD gene was isolated from a cosmid gene bank by complementation of an S. typhimurium aroD mutant. Analysis of the DNA sequence revealed the presence of an open reading frame capable of encoding a protein of 252 amino acids with a calculated Mr of 27706. Comparison of the deduced S. typhi 3-dehydroquinase protein sequence with that elucidated for E. coli revealed 69% homology. Alignment of the S. typhi sequence and equivalent Aspergillus nidulans and Saccharomyces cerevisiae sequences showed that homology was lower, at 24%, but still significant. Use of a minicell expression system demonstrated that a polyclonal antibody raised against E. coli 3-dehydroquinase cross-reacted with its S. typhi counterpart. en_US
dc.language en_US
dc.publisher Soc General Microbiology en_US
dc.title Molecular cloning and characterization of the aroD gene encoding 3-dehydroquinase from Salmonella typhi en_US
dc.parent Journal Of General Microbiology en_US
dc.journal.volume 137 en_US
dc.journal.number 1 en_US
dc.publocation Reading en_US
dc.identifier.startpage 147 en_US
dc.identifier.endpage 152 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000061398 en_US
dc.personcode 0000061422 en_US
dc.personcode 0000061754 en_US
dc.personcode 0000061755 en_US
dc.personcode 0000061756 en_US
dc.personcode 0000061511 en_US
dc.personcode 0000061403 en_US
dc.personcode 0000061423 en_US
dc.personcode 109028 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid en_US
dc.staffid 109028 en_US


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