Isolation And Computer-Aided Characterization Of Mmei, A Type-Ii Restriction Endonuclease From Methylophilus-Methylotrophus

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dc.contributor.author Boyd, Anthony en_US
dc.contributor.author Charles, Ian en_US
dc.contributor.author Keyte, Jw en_US
dc.contributor.author Brammar, Wj en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:21:13Z
dc.date.available 2011-02-07T06:21:13Z
dc.date.issued 1986 en_US
dc.identifier 2009002875 en_US
dc.identifier.citation Boyd Anthony et al. 1986, 'Isolation And Computer-Aided Characterization Of Mmei, A Type-Ii Restriction Endonuclease From Methylophilus-Methylotrophus', Oxford Univ Press United Kingdom, vol. 14, no. 13, pp. 5255-5274. en_US
dc.identifier.issn 0305-1048 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13386
dc.description.abstract A Type II restriction endonuclease, MmeI, has been purified from the obligate methylotroph, Methylophilus methylotrophus. The enzyme was shown to have the non-palindromic recognition sequence 5'-T C C Pu A C (N)20-3', 3'-A G G Py T G (N)18-5' and to cleave (as indicated) on the 3' side, generating a two nucleotide 3' projection. Determination of the recognition sequence was achieved using two new computer programs; RECOG, which predicts recognition sequences from the pattern of restriction fragments obtained from DNAs of known sequence, and GELSIM, which generates graphical simulations of DNA band patterns obtained by gel electrophoresis of restriction digests of sequenced DNA molecules. en_US
dc.language en_US
dc.publisher Oxford Univ Press United Kingdom en_US
dc.relation.isbasedon http://dx.doi.org/10.1093/nar/14.13.5255 en_US
dc.title Isolation And Computer-Aided Characterization Of Mmei, A Type-Ii Restriction Endonuclease From Methylophilus-Methylotrophus en_US
dc.parent Nucleic Acids Research en_US
dc.journal.volume 14 en_US
dc.journal.number 13 en_US
dc.publocation Oxford en_US
dc.identifier.startpage 5255 en_US
dc.identifier.endpage 5274 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 060100 en_US
dc.personcode 940308 en_US
dc.personcode 109028 en_US
dc.personcode 0000060129 en_US
dc.personcode 0000060130 en_US
dc.percentage 100 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:A1986D269500010 en_US
dc.description.keywords NA en_US
dc.staffid en_US


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