The commercially available restriction enzyme BspHl is blocked by overlapping methylation

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dc.contributor.author Servos, Spiros en_US
dc.contributor.author Silva, C. en_US
dc.contributor.author Dougan, Gordon en_US
dc.contributor.author Charles, Ian en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:20:52Z
dc.date.available 2011-02-07T06:20:52Z
dc.date.issued 1991 en_US
dc.identifier 2009005030 en_US
dc.identifier.citation Servos Spiros et al. 1991, 'The commercially available restriction enzyme BspHl is blocked by overlapping methylation', Oxford Univ Press United Kingdom, vol. 19, no. 1, pp. 183-183. en_US
dc.identifier.issn 0305-1048 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13341
dc.description.abstract The restriction endonuclease Bspill. conunercially available from New England Biolabs (Bishop's Stortford, UK), cleaves the site T/CATGA. This restriction enzyme is of particular use in the construction of gene expression cassettes in the vector pKK233 -2 (I) as it is one of the three enzymes along with AflIII and NcoI that has an 'ATG' in its recognition sequence. In this paper we show that BspHI is Dam methylase (2) sensitive and will not cleave DNA that has the overlapping sequ~nce GATC. The oligonucleotides: OS3142 5' -CTAGATCATGAGCGAACTG~ TCGTGAACGTGATCAAAGCTTGGTAC- 3' and OS3143 5'-CAAGCTTTGATCACGTTCACGATCAGTTCGCTCATGAT- 3' were annealed and ligated into XbaI-KpnI digested bluescript SKI1+ (Stratagene, Cambridge, UK}. en_US
dc.language en_US
dc.publisher Oxford Univ Press United Kingdom en_US
dc.relation.isbasedon http://dx.doi.org/10.1093/nar/19.1.183 en_US
dc.title The commercially available restriction enzyme BspHl is blocked by overlapping methylation en_US
dc.parent Nucleic Acids Research en_US
dc.journal.volume 19 en_US
dc.journal.number 1 en_US
dc.publocation Oxford en_US
dc.identifier.startpage 183 en_US
dc.identifier.endpage 183 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 060100 en_US
dc.personcode 0000061398 en_US
dc.personcode 0000061997 en_US
dc.personcode 0000061403 en_US
dc.personcode 109028 en_US
dc.percentage 34 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid en_US
dc.staffid 109028 en_US


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