Identification of lipoprotein MsIA as a neoteric virulence factor of Mycoplasma gallisepticum

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dc.contributor.author Szczepanek S en_US
dc.contributor.author Frasca, Jr. S. en_US
dc.contributor.author Schumacher V. en_US
dc.contributor.author Liao Xiaofen en_US
dc.contributor.author Padula Matthew en_US
dc.contributor.author Djordjevic Steven en_US
dc.contributor.author Geary S. en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:20:49Z
dc.date.available 2011-02-07T06:20:49Z
dc.date.issued 2010 en_US
dc.identifier 2009006776 en_US
dc.identifier.citation Szczepanek S et al. 2010, 'Identification of lipoprotein MsIA as a neoteric virulence factor of Mycoplasma gallisepticum', Amer Soc Microbiology, vol. 78, no. 8, pp. 3475-3483. en_US
dc.identifier.issn 0019-9567 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/13335
dc.description.abstract Many lipoproteins are expressed on the surfaces of mycoplasmas, and some have been implicated as playing roles in pathogenesis. Family 2 lipoproteins of Mycoplasma pneumolliae have a conserved "mycoplasma lipoprotein X" central domain and a "mycoplasma lipoprotein 10" C-terminal domain and are dill'erentially expressed in response to environmental conditions. Homologues of family 2 lipoproteins are Mycoplasma specific and include the lipoprotein of Mycoplasma gallisepticum, encoded by the MGA0674 gene. Comparative transcriptomic analysis of the M. gallisepticum live attenuated vaccine strain F and the virulent strain R1ow, reported in this study, indicated that MGA0674 is one of several dili'erentially expressed genes. The MGA0674- encoded lipoprotein is a proteolytically processed, immunogenic, TX-1l4 detergent-phase protein which appears to have antigenic divergence between field strains Rtow and S6. We examined the virulence of an R10w aMGA0674 mutant (PIH9) in vivo and observed reduced recovery and attenuated virulence in the tracheas of experimentally infected chickens. The virulence of two additional R10w aMGA0674mutauts, 2162 and 2204, was assessed in a second in vivo virulence experiment. These mutants exhibited partial to complete attenuation ill vivo, but recovery was observed more frequently. Since only Mycoplasma species harbor homologues of MGA0674, the gene product has been renamed "Mycoplasma-specific lipoprotein A" (MsIA). Collectively, these data indicate that MslA is an immunogenic lipoprotein exhibiting reduced expression in an attenuated strain and plays a role in M. gallisepticllm virulence. en_US
dc.language en_US
dc.publisher Amer Soc Microbiology en_US
dc.relation.isbasedon http://dx.doi.org/10.1128/IAI.00154-10 en_US
dc.title Identification of lipoprotein MsIA as a neoteric virulence factor of Mycoplasma gallisepticum en_US
dc.parent Infection And Immunity en_US
dc.journal.volume 78 en_US
dc.journal.number 8 en_US
dc.publocation Washington DC, USA en_US
dc.identifier.startpage 3475 en_US
dc.identifier.endpage 3483 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000062968;0000063282;0000063283;0000052318;970082;107126;0000063284 en_US
dc.percentage 000050 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid en_US


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