Quantifying microbial diversity: morphotypes, 16S rRNA genes, and carotenoids of oxygenic phototrophs in microbial mats

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dc.contributor.author Nubel, Ulrich en_US
dc.contributor.author Garcia-Pichel, Ferran en_US
dc.contributor.author Kuhl, Michael en_US
dc.contributor.author Muyzer, Gerard en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:20:28Z
dc.date.available 2011-02-07T06:20:28Z
dc.date.issued 1999 en_US
dc.identifier 2009005736 en_US
dc.identifier.citation Nubel Ulrich et al. 1999, 'Quantifying microbial diversity: morphotypes, 16S rRNA genes, and carotenoids of oxygenic phototrophs in microbial mats', American Society for Microbiology, vol. 65, no. 2, pp. 422-430. en_US
dc.identifier.issn 0099-2240 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13290
dc.description.abstract We quantified the diversity of oxygenic phototrophic microorganisms present in eight hypersaline microbial mats on the basis of three cultivation-independent approaches. Morphological diversity was studied by microscopy. The diversity of carotenoids was examined by extraction from mat samples and high-pressure liquid chromatography analysis. The diversity of 16S rRNA genes from oxygenic phototrophic microorganisms was investigated by extraction of total DNA from mat samples, amplification of 16S rRNA gene segments from cyanobacteria and plastids of eukaryotic algae by phylum-specific PCR, and sequence-dependent separation of amplification products by denaturing-gradient gel electrophoresis. A numerical approach was introduced to correct for crowding the results of chromatographic and electrophoretic analyses. Diversity estimates typically varied up to twofold among mats. en_US
dc.language en_US
dc.publisher American Society for Microbiology en_US
dc.relation.isbasedon NA en_US
dc.title Quantifying microbial diversity: morphotypes, 16S rRNA genes, and carotenoids of oxygenic phototrophs in microbial mats en_US
dc.parent Applied and Environmental Microbiology en_US
dc.journal.volume 65 en_US
dc.journal.number 2 en_US
dc.publocation Washington DC, USA en_US
dc.identifier.startpage 422 en_US
dc.identifier.endpage 430 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000062158 en_US
dc.personcode 0000062479 en_US
dc.personcode 107129 en_US
dc.personcode 0000061198 en_US
dc.percentage 50 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid en_US


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