A High Production-Rate Of Translatable IgG Messenger-RNA Accounts For The Amplified Synthesis Of IgG In Myeloma Cells

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dc.contributor.author Wallach, Michael en_US
dc.contributor.author Laskov, R en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:20:22Z
dc.date.available 2011-02-07T06:20:22Z
dc.date.issued 1980 en_US
dc.identifier 2008005991 en_US
dc.identifier.citation Wallach Michael and Laskov R 1980, 'A High Production-Rate Of Translatable IgG Messenger-RNA Accounts For The Amplified Synthesis Of IgG In Myeloma Cells', Springer Verlag, vol. 110, no. 2, pp. 545-554. en_US
dc.identifier.issn 0014-2956 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13276
dc.description.abstract The aim of the present work was to determine whether the accumulation of Ig mRNA in myeloma cells is due to a high rate of production or to a high stability of these molecules. Specific mRNAs for the light and heavy polypeptide chains of IgG were isolated from the murine MPC-11 myeloma tumor cells by immune precipitation of polysomes which synthesize these chains. It was found that the immune-precipitated polysomes were enriched 10--30-fold in the gamma and chi mRNA sequences respectively. In the wheat germ cell-free system the chi mRNA preparation was translated mainly into three polypeptides of Mr 25 000, 18 000, and 15 000. The method of immune precipitation of polysomes was also used to characterize three variant clones of MPC-11 myeloma. It was found that little if any gamma-chain polysomes are present in the L-chain producer and non-producer clones, while a substantial amount of chi-chain polysomes was present in the non-producer clone. This may be due to the presence in the non-producer cells of the constant region chi-chain fragment. In order to determine the relative synthesis rate of chi and gamma mRNAs, pulse-labelled polysomes were immune precipitated using antibodies to chi and gamma chains. It was found that chi and gamma mRNA molecules are produced at a very high relative rate each accounting for 10--15% of the total labeled mRNA after 1 h of labeling. These values are higher than the steady-state pool size of chi and gamma mRNA, which was 5--6%, and indicates that the half-life of these molecules is not unusually high. It is concluded that the amplified synthesis of immunoglobulin chains in myeloma cells is mainly due to a high rate of production of translatable chi and gamma mRNAs en_US
dc.language en_US
dc.publisher Springer Verlag en_US
dc.relation.isbasedon http://dx.doi.org/10.1111/j.1432-1033.1980.tb04897.x en_US
dc.title A High Production-Rate Of Translatable IgG Messenger-RNA Accounts For The Amplified Synthesis Of IgG In Myeloma Cells en_US
dc.parent F E B S Journal en_US
dc.journal.volume 110 en_US
dc.journal.number 2 en_US
dc.publocation New York en_US
dc.identifier.startpage 545 en_US
dc.identifier.endpage 554 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060100 en_US
dc.personcode 021128 en_US
dc.personcode 0000051572 en_US
dc.percentage 40 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:A1980KN87200028 en_US
dc.description.keywords en_US
dc.staffid en_US

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