Molecular-Cloning And Characterization Of Protective Outer-Membrane Protein-P.69 From Bordetella-Pertussis

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dc.contributor.author Charles, Ian en_US
dc.contributor.author Dougan, G en_US
dc.contributor.author Pickard, D en_US
dc.contributor.author Chatfield, S en_US
dc.contributor.author Smith, Michael en_US
dc.contributor.author Novotny, P en_US
dc.contributor.author Morrissey, P en_US
dc.contributor.author Fairweather, Nf en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:20:02Z
dc.date.available 2011-02-07T06:20:02Z
dc.date.issued 1989 en_US
dc.identifier 2009002881 en_US
dc.identifier.citation Charles Ian et al. 1989, 'Molecular-Cloning And Characterization Of Protective Outer-Membrane Protein-P.69 From Bordetella-Pertussis', Natl Acad Sciences, vol. 86, no. 10, pp. 3554-3558. en_US
dc.identifier.issn 0027-8424 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13236
dc.description.abstract Protein P.69 is localized on the outer membrane of Bordetella pertussis and is one of the virulence factors believed to contribute to the disease state of whooping cough. We demonstrate that protein synthesis of P.69 is under genetic control of the vir locus. Using oligonucleotide probes derived from the protein sequence of a cyanogen bromide fragment, we have cloned the gene for P.69 from B. pertussis CN2992. Analysis of the DNA sequence reveals a G + C-rich gene capable of encoding a protein of 910 amino acids with a Mr of 93,478, suggesting that P.69 is a processed form of a larger precursor. In common with some of the genes in the pertussis toxin operon, the sequence CCTGG was found 5' to the ATG initiation codon. At the 3' end, 29 bases after the TAA stop codon, the sequence GTTTTTCCT was found and may have some function in transcription termination. A full-length clone of the gene for P.69 carried by the cosmid pBPI69 was unable to direct the expression of P.69 protein in an Escherichia coli host. The generation of P.69-fusion products allowed the detection of P.69-specific protein products synthesized in E. coli. en_US
dc.language en_US
dc.publisher Natl Acad Sciences en_US
dc.relation.isbasedon http://www.pnas.org/content/86/10/3554.abstract?sid=3d255ff6-dad0-4af6-8394-c0811bbbebeb en_US
dc.title Molecular-Cloning And Characterization Of Protective Outer-Membrane Protein-P.69 From Bordetella-Pertussis en_US
dc.parent Proceedings Of The National Academy Of Sciences Of The United States Of America en_US
dc.journal.volume 86 en_US
dc.journal.number 10 en_US
dc.publocation Washington en_US
dc.identifier.startpage 3554 en_US
dc.identifier.endpage 3558 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 060409 en_US
dc.personcode 109028 en_US
dc.personcode 0000060138 en_US
dc.personcode 0000060143 en_US
dc.personcode 0000060141 en_US
dc.personcode 100304 en_US
dc.personcode 0000060144 en_US
dc.personcode 0000046206 en_US
dc.personcode 0000060145 en_US
dc.percentage 100 en_US
dc.classification.name Molecular Evolution en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:A1989U652300025 en_US
dc.description.keywords NA en_US


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