Gene expression profiling of HUH7-ins Lack of a granulogenic function for Chromogranin A

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dc.contributor.author Lutherborrow, Mark en_US
dc.contributor.author Appavoo, Mathiyalagan en_US
dc.contributor.author Simpson, Ann en_US
dc.contributor.author Tuch, Bernard en_US
dc.contributor.editor en_US
dc.date.accessioned 2011-02-07T06:18:21Z
dc.date.available 2011-02-07T06:18:21Z
dc.date.issued 2009 en_US
dc.identifier 2009004540 en_US
dc.identifier.citation Lutherborrow Mark et al. 2009, 'Gene expression profiling of HUH7-ins Lack of a granulogenic function for Chromogranin A', Landes Bioscience, vol. 1, no. 1, pp. 60-72. en_US
dc.identifier.issn 1938-2014 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13048
dc.description.abstract Previously, the insulin producing liver cell line HUH7-ins has been shown to synthesize, store and secrete insulin in response to glucose via secretory granules. The current study characterized the gene expression profile of HUH7-ins with the aim to identify changes possibly involved in the formation of granules. Additionally, experiments were conducted to determine the influence of chromograninA (egA) on secretory granule biogenesis (5GB) in HUH7-ins. Expression of 165 genes were significantly changed in HUH7-ins, though interestingly the majority of secretory granule associated genes, such as the chromogranins were unchanged. egA was over-expressed in glucose unresponsive HUH7-ins cells to test whether egA played a role in 5GB and would restore the regulated secretory phenotype. Over expression affected neither the storage nor regulated secretion of insulin. These data suggest that 5GB may by regulated at a post-transcriptional level with no evidence to indicate that egA regulates SGB in the cell line HUH7-ins. en_US
dc.language en_US
dc.publisher Landes Bioscience en_US
dc.relation.isbasedon http://dx.doi.org/10.4161/isl.1.1.8994 en_US
dc.title Gene expression profiling of HUH7-ins Lack of a granulogenic function for Chromogranin A en_US
dc.parent Islets en_US
dc.journal.volume 1 en_US
dc.journal.number 1 en_US
dc.publocation USA en_US
dc.identifier.startpage 60 en_US
dc.identifier.endpage 72 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 020201 en_US
dc.personcode 0000024913 en_US
dc.personcode 0000061582 en_US
dc.personcode 940084 en_US
dc.personcode 100016 en_US
dc.percentage 100 en_US
dc.classification.name Atomic and Molecular Physics en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Secrerory granule biogenesis, microarray, chromogranin A, regulated secretion en_US


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