Lysozyme Interactions with poly(HEMA)-based Hydrogel

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dc.contributor.author Lord, Megan en_US
dc.contributor.author Stenzel, Martina en_US
dc.contributor.author Simmons, Anne en_US
dc.contributor.author Milthorpe, Bruce en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-07-15T07:27:21Z
dc.date.available 2010-07-15T07:27:21Z
dc.date.issued 2006 en_US
dc.identifier 2008002383 en_US
dc.identifier.citation Lord Megan et al. 2006, 'Lysozyme Interactions with poly(HEMA)-based Hydrogel', Elsevier Inc, vol. 27, no. 8, pp. 1341-1345. en_US
dc.identifier.issn 0142-9612 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/12813
dc.description.abstract Lysozyme interaction with an acrylic-based hydrogel, poly(2-hydroxyethyl methacrylate) co-methacrylic acid (P(HEMA-MAA)), was investigated using a combination of quartz crystal microbalance with dissipation (QCM-D), surface plasmon resonance (SPR) and dual polarisation interferometry (DPI). This combination of techniques demonstrated that lysozyme initially absorbed into the hydrogel matrix and displaced water from the hydrogel while subsequent lysozyme additions were adsorbed onto the surface of the hydrogel material. QCM-D, being sensitive to bound water, showed an overall decrease in mass and stiffening of the layer after lysozyme addition. SPR, a water insensitive technique, showed a net mass increase after addition of lysozyme and buffer rinses. DPI showed that the first exposure of lysozyme to P(HEMA-MAA) was consistent with lysozyme absorption while subsequent lysozyme exposures were consistent with lysozyme adsorption. en_US
dc.language en_US
dc.publisher Elsevier Inc en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/j.biomaterials.2005.09.007 en_US
dc.title Lysozyme Interactions with poly(HEMA)-based Hydrogel en_US
dc.parent Biomaterials en_US
dc.journal.volume 27 en_US
dc.journal.number 8 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 1341 en_US
dc.identifier.endpage 1345 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 090300 en_US
dc.personcode 0000048911 en_US
dc.personcode 0000048933 en_US
dc.personcode 0000048915 en_US
dc.personcode 105631 en_US
dc.percentage 100 en_US
dc.classification.name Biomedical Engineering en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Hydrogels; Protein adsorption; Lysozyme; Contact lens en_US
dc.staffid 105631 en_US


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