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Browsing by Author "Stokes Harold"

Now showing items 1-20 of 72

ACID: annotation of cassette and integron data

Joss M; Koenig Jeremy; Labbate Maurizio; Polz Martin; Gillings Michael; Stokes Harold; Doolittle W; Boucher Yan (BioMed Central Ltd, 2009)

Although integrons and their associated gene cassettes are present in ~10% of bacteria and can represent up to 3% of the genome in which they are found, very few have been properly identified and annotated in public ...
Are humans increasing bacterial evolvability?

Gillings Michael; Stokes Harold (Elsevier Limited, 2012)

Attempts to control bacterial pathogens have led to an increase in antibiotic-resistant cells and the genetic elements that confer resistance phenotypes. These cells and genes are disseminated simultaneously with the ...
Binding of the purified integron DNA integrase Intl1 to integron- and cassette-associated recombination sites

Collis Christina; Kim Minah; Stokes Harold; Hall R (Blackwell Science Ltd, 1998)

The site-specific recombinase lntl1, encoded by class 1 integrons, catalyses the integration and excision of gene cassettes by recognizing two classes of sites, the integron-associated attl1 site and the 59-base element ...
Blood protein purification and simulaneous removal of nonenveloped viruses using tangential-flow preparative electrophoresis

Evtushenko Michael; Wang Karen; Stokes Harold; Nair Hari (Wiley-V C H Verlag Gmbh, 2005)

Gradiflow is new technology allowing purification of important blood proteins from viral contaminated plasma. Protein purification is based on unique scalable tangential-flow preparative electrophoresis, and is distinct ...
Characterisation of a chloramphenicol acetyltransferase determinant found in the chromosome of Pseudomonas aeruginosa

White Peter; Stokes Harold; Bunny Kim; Hall Ruth (Elsevier Inc, 1999)

The open reading frame (ORF) in the Pseudomonas aeruginosa chromosome, whose product resembles the chloramphenicol acetyltransferases (CAT) belonging to the CATB family, was cloned and shown to confer resistance to ...
Characterization of the Class 3 Integron and the Site-Specific Recombination System It Determines

Collis Christina; Kim Mi-Jurng; Partridge S; Stokes Harold; Hall Ruth (Amer Soc Microbiology, 2002)

Integrons capture gene cassettes by using a site-specific recombination mechanism. As only one class of integron and integron-determined site-specific recombination system has been studied in detail, the properties of a ...
Class 1 integron-associated spread of resistance regions in Pseudomonas aeruginosa: plasmid or chromosomal platforms?

Stokes Harold; Martinez Diaz Maria; Bhattacharya Piklu; Djordjevic Steven (Oxford University Press, 2012)

Multidrug-resistant Pseudomonas aeruginosa infections are a growing clinical problem. Of particular concern is the range of b-lactamase genes associated with this species. If the spread of resistance is to be controlled, ...
A Class 1 Integron Present in a Human Commensal Has a Hybrid Transposition Module Compared to Tn402: Evidence of Interaction with Mobile DNA from Natural Environments

Labbate Maurizio; Chowdhury Piklu; Stokes Harold (Amer Soc Microbiology, 2008)

In a survey of class 1 integrons from human stools, an unusual class 1 integron from a strain of Enterobacter cloacae was isolated and characterized in detail. Sequence analysis of a fosmid containing the class 1 integron ...
Class 1 Integrons In Benthic Bacterial Communities: Abundance, Association With Tn402-Like Transposition Modules And Evidence For Coselection With Heavy-Metal Resistance

Rosewarne Cp; Pettigrove V; Stokes Harold; Parsons Yvonne (Wiley-Blackwell Publishing, Inc, 2010)

The integron/gene cassette system contributes to lateral gene transfer of genetic information in bacterial communities, with gene cassette-encoded proteins potentially playing an important role in adaptation to stress. ...
Class 1 Integrons Potentially Predating the Association with Tn402-Like Transposition Genes Are Present in a Sediment Microbial Community

Stokes Harold; Nesbo Camilla; Holley Marita; Balh Martin; Gillings Michael; Boucher Yan (Amer Soc Microbiology, 2006)

Integrons are genetic elements that contribute to lateral gene transfer in bacteria as a consequence of possessing a site-specific recombination system. This system facilitates the spread of genes when they are part of ...
Comparison of Plasmids in Strains of Zymomonas mobilis

Stokes Harold; Dally E; Yablonsky M; Eveleigh D (Academic Press, 1983)

Four strains of Zymomonas mobilis were examined for their resistance to antimicrobial agents and found to have similar resistance profiles. Plasmid DNA was extracted and purified by CsCl dye-buoyant density centrifugation; ...
Complementation Analysis in Pseudomonas aeruginosa of the Transfer Genes of the Wide Host Range R Plasmid RI8

Stokes Harold; Moore R; Krishnapillai V (Academic Press, 1981)

A method of transductional complementation was developed in Pseudomonas aeruginosa to identify the cistrons involved in the conjugal transfer of the wide host range R plasmid R18. This used the P. aeruginosa bacteriophage ...
Coral-mucus-associated Vibrio integrons in the Great Barrier Reef: genomic hotspots for environmental adaptation

Koenig Jeremy; Bourne David; Curtis Bruce; Dlutek Marlena; Stokes Harold; Doolittle W; Boucher Yan (Nature Publishing Group, 2011)

Integron cassette arrays in a dozen cultivars of the most prevalent group of Vibrio isolates obtained from mucus expelled by a scleractinian coral (Pocillopora damicornis) colony living on the Great Barrier Reef were ...
Crystal structure of an integron gene cassette- associated protein from Vibrio cholerae identifies a cationic drug-binding module

Deshpande Chandrika; Harrop Stephen; Boucher Yan; Hassan Karl; Di Leo Rosa; Xu Xiaohui; Cui Hong; Savchenko Alexei; Chang Changsoo; Labbate Maurizio; Paulsen Ian; Stokes Harold; Curmi Paul; Mabbutt Bridget (Public library of Science, 2011)

Background: The direct isolation of integron gene cassettes from cultivated and environmental microbial sources allows an assessment of the impact of the integron/gene cassette system on the emergence of new phenotypes, ...
Definition of the attI1 site of class 1 integrons

Partridge S; Recchia Gavin; Scaramuzzi Carol; Collis Christina; Stokes Harold; Hall Ruth (Society for General Microbiology, 2000)

Integron-encoded integrases recognize two distinct types of recombination site: attI sites, found in integrons, and members of the 59-base element (59-be) family, found in the integron-associated gene cassettes. The class ...
Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries: a comparative study

Roy Chowdhury Piklu; Ingold Ana; Vanegas-Gomez Natasha; Martinez Diaz Maria; Merlino John; Merkier Andrea; Castro Mercedes; Rocha Gerardo; Borthagaray Graciela; Centron Daniela; Toledo Helia; Marquez Carolina; Stokes Harold (American Society for Microbiology, 2011)

A comparative genetic analysis of 42 clinical Klebsiella pneumoniae isolates, resistant to two or more antibiotics belonging to the broad-spectrum -lactam group, sourced from Sydney, Australia, and three South American ...
Diverse mobilized class 1 integrons are common in the chromosomes of pathogenic Pseudomonas aeruginosa clinical isolates

Martinez Diaz Maria; Marquez Carolina; Ingold Ana; Merlino John; Djordjevic Steven; Stokes Harold; Bhattacharya Piklu (American Society for Microbiology, 2012)

Eleven clinical class 1 integron-containing Pseudomonas aeruginosa isolates from Australia and Uruguay were investigated for the genomic locations of these elements. Several novel class 1 integrons/transposons were found ...
Efficiency of Recombination Reactions Catalyzed by Class 1 Integron Integrase IntI1

Collis Christina; Recchia Gavin; Kim Mi-Jurng; Stokes Harold; Hall Ruth (Amer Soc Microbiology, 2001)

The class 1 integron integrase, IntI1, recognizes two distinct types of recombination sites, attI sites, found in integrons, and members of the 59-be family, found in gene cassettes. The efficiencies of the integrative ...
Evidence for dynamic exchange of qac gene cassettes between class 1 integrons and other integrons in freshwater biofilms

Gillings Michael; Holley Marita; Stokes Harold (Wiley-Blackwell Publishing, Inc, 2009)

Class 1 integrons carried by pathogens have acquired over 100 different gene cassettes encoding resistance to antimicrobial compounds, helping to generate a crisis in the management of infectious disease. It is presumed ...
The Evolution of Class 1 Integrons and the Rise of Antibiotic Resistance

Gillings Michael; Boucher Yan; Labbate Maurizio; Holmes Andrew; Krishnan Samyuktha; Holley Marita; Stokes Harold (Amer Soc Microbiology, 2008)

Class 1 integrons are central players in the worldwide problem of antibiotic resistance, because they can capture and express diverse resistance genes. In addition, they are often embedded in promiscuous plasmids and ...

Now showing items 1-20 of 72

Browsing by Author "Stokes Harold"

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Browsing by Author "Stokes Harold"